Thermo Fisher Scientific Phospho-XRCC4 (Ser260) Polyclonal Antibody
XRCC4 단백질의 Ser260 인산화 형태를 특이적으로 인식하는 폴리클로날 항체. Western blot 및 IHC(파라핀 절편)에서 사용 가능. 사람, 생쥐, 랫드 반응성. 액상 형태로 PBS/glycerol buffer에 보관. 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human XRCC4 (Accession Q13426), corresponding to amino acid residues around phosphorylated Ser260 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2903224 |
Product Specific Information
This antibody detects endogenous levels of XRCC4 only when phosphorylated at Ser260.
Target Information
The x-ray repair cross-complementing (XRCC) proteins are responsible for efficiently repairing and maintaining genetic stability following DNA base damage. These genes share sequence similarity with the yeast DNA repair protein Rad51.
XRCC4 is an accessory factor of DNA ligase IV that preferentially binds DNA with nicks or broken ends. It binds to DNA ligase IV and enhances its joining activity, and it is also involved in V(D)J recombination.
Defects in any component of the DNA repair/V(D)J recombination machinery (Ku-70, Ku-80, DNA-PKCS, XRCC4, DNA ligase IV) can lead to failure of V(D)J rearrangement and early block in both T and B cell maturation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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