Thermo Fisher Scientific IL-4 Monoclonal Antibody (11B11), Alexa Fluor 488, eBioscience

Thermo Fisher Scientific IL-4 Monoclonal Antibody (11B11), Alexa Fluor 488, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• APC
• Brilliant UV 615
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 786
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation available

Product Specific Information

The 11B11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kD cytokine. It is a neutralizing antibody. Mouse IL-4 (also known as BCDF, BCGF-1, BSF-1) is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. IL-4 promotes proliferation and differentiation of B cells and induces Ig isotype switching along with upregulation of MHC class II and CD23.

Applications Reported:
Used for intracellular staining followed by flow cytometric analysis.

Applications Tested:
Validated for intracellular staining and flow cytometric analysis of Th2-polarized, stimulated mouse splenocytes. Recommended ≤1 µg/test (100 µL final volume). Optimal titration recommended.

Excitation/Emission: 488 nm / 519 nm
Laser: Blue
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells and acts as a ligand for the IL-4 receptor. The receptor also binds IL13, contributing to overlapping functions. STAT6 mediates IL-4 signaling. The IL-4 gene is part of a cytokine cluster on chromosome 5q, near IL13, IL3, IL5, and CSF2, coordinated by long-range regulatory elements. IL-4 delta 2 is a known splice variant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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