Thermo Fisher Scientific IL-4 Monoclonal Antibody (11B11), Alexa Fluor 488, eBioscience
마우스 IL-4를 인식하는 중화용 단일클론 항체로 Alexa Fluor 488 형광 표지. Flow cytometry 및 intracellular staining에 적합. 0.5 mg/mL 농도, PBS 버퍼 및 sodium azide 포함. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific IL-4 Monoclonal Antibody (11B11), Alexa Fluor 488, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 1 µg/test | View 38 publications |
| ELISA | - | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), Alexa Fluor™ 488, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 11B11 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_469921 |
Available Formats
- Unconjugated
- APC
- Brilliant UV 615
- Brilliant Violet 421
- Brilliant Violet 480
- Brilliant Violet 786
- Functional Grade
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Request custom conjugation available
Product Specific Information
The 11B11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kD cytokine. It is a neutralizing antibody. Mouse IL-4 (also known as BCDF, BCGF-1, BSF-1) is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. IL-4 promotes proliferation and differentiation of B cells and induces Ig isotype switching along with upregulation of MHC class II and CD23.
Applications Reported:
Used for intracellular staining followed by flow cytometric analysis.
Applications Tested:
Validated for intracellular staining and flow cytometric analysis of Th2-polarized, stimulated mouse splenocytes. Recommended ≤1 µg/test (100 µL final volume). Optimal titration recommended.
Excitation/Emission: 488 nm / 519 nm
Laser: Blue
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells and acts as a ligand for the IL-4 receptor. The receptor also binds IL13, contributing to overlapping functions. STAT6 mediates IL-4 signaling. The IL-4 gene is part of a cytokine cluster on chromosome 5q, near IL13, IL3, IL5, and CSF2, coordinated by long-range regulatory elements. IL-4 delta 2 is a known splice variant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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