Thermo Fisher Scientific Human IFN-alpha A (alpha 2a) (Carrier-Free) Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

E. coli

Molecular weight

19.2 kDa

Class

Recombinant

Type

Protein

Purity

>95%

Endotoxin concentration

<1 EU/µg

Activity

EC50 ~ 1 U/mL; determined by use of the cytopathic effect inhibition assay described in [Rubinstein, S. et al. (1981). J. Virol. 37, 755-758]; [Familletti, P.C. et al. (1981). Methods in Enzymology, Vol. 78, 387-394]. The units are determined with respect to the international reference standard for human interferon alpha A (Hu-IFN-alpha-A) provided by the National Institutes of Health [see Pestka, S. (1986). Methods in Enzymology, Academic Press, New York 119, 14-23]. Unit of activity measured on bovine MDBK cells with vesicular stomatitis virus (VSV).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

purified

Storage buffer

PBS

Contains

no preservative

Storage conditions

-80° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

After receipt, the product may be stored at -20°C for short-term use (less than 6 months). For long-term storage, we recommend storing the product at -70°C or below for retention of full activity. Thaw product vial by incubation in cold tap water until just thawed. Aliquot and refreeze unused contents to minimize effects of freeze thawing. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of product should be in buffers containing protein such as 0.1% BSA or tissue culture media with serum. Dilution for freezing of material below 2 x 10^5 units/mL is not recommended. One freeze thaw cycle is equivalent to thawing an aliquot prepared from the material received.

Target Information

Human Interferon-alpha 2a produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19241 Dalton. The Interferon-a 2a gene was obtained from human leukocytes. The rHuIFN-a 2a is purified by proprietary chromatographic techniques.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.