
Thermo Fisher Scientific Human IFN-alpha A (alpha 2a) (Carrier-Free) Recombinant Protein
Human IFN-alpha A (alpha 2a) 재조합 단백질로, E. coli에서 발현된 고순도(>95%) 캐리어-프리 형태. 세포 보호 활성 검증 완료. ELISA, WB, IP 등 다양한 연구용 응용에 적합. -80°C에서 안정적으로 보관 가능.
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Applications
- Western Blot (WB): Assay-dependent
- ELISA: Assay-dependent
- Immunoprecipitation (IP): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Molecular Weight | 19.2 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >95% |
| Endotoxin Concentration | <1 EU/µg |
| Activity | EC50 ~ 1 U/mL; determined by cytopathic effect inhibition assay (Rubinstein et al., 1981; Familletti et al., 1981). Units referenced to NIH standard (Pestka, 1986). Activity measured on bovine MDBK cells with vesicular stomatitis virus (VSV). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -80°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Dry ice |
Product Specific Information
- Upon receipt, product may be stored at -20°C for short-term use (less than 6 months).
- For long-term storage, store at -70°C or below to maintain full activity.
- Thaw vial in cold tap water until just thawed.
- Aliquot and refreeze unused contents to minimize freeze-thaw effects.
- Refreeze on dry ice or in a dry ice/alcohol bath.
- Further dilution should be done in buffers containing protein (e.g., 0.1% BSA) or tissue culture media with serum.
- Do not dilute below 2 × 10⁵ units/mL for freezing.
- One freeze-thaw cycle equals thawing an aliquot prepared from the received material.
Target Information
Human Interferon-alpha 2a produced in E. coli is a single, non-glycosylated polypeptide chain containing 165 amino acids with a molecular mass of 19,241 Da.
The Interferon-α 2a gene was obtained from human leukocytes.
Recombinant Human IFN-α 2a is purified using proprietary chromatographic techniques.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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