Thermo Fisher Scientific Human IFN-alpha A (alpha 2a) Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 5 publications 5 publications

Product Specifications

Species

Human

Published species

Not Applicable

Expression System

E. coli

Molecular weight

19.2 kDa

Class

Recombinant

Type

Protein

Activity

EC50 ~ 1 U/mL; determined by use of the cytopathic effect inhibition assay described in [Budd et al. (1985) Canc. Chem. Pharm. 12:39]. The units are determined by use of a Human IFN Alpha A (Hu IFN- alpha A [2a]) laboratory standard calibrated to the international reference standard for Human Interferon Alpha A provided by the National Institutes of Health [Meager et al. (2001) J. Immunol Methods. 257(1-2):17-33].

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Lot-specific

Purification

purified

Storage buffer

PBS with 0.1% BSA

Contains

no preservative

Storage conditions

-80° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

Store at -70°C or below for retention of full activity. Thaw product vial by incubation in cold tap water until just thawed. Aliquot and refreeze unused contents to minimize effects of freeze thawing. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of product should be in buffers containing protein such as 0.1% BSA or tissue culture media with serum. Dilution for freezing of material below 2 x 10^5 units/mL is not recommended. One freeze thaw cycle is equivalent to thawing an aliquot prepared from the material received.

Target Information

Human Interferon-alpha 2a produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19241 Dalton. The Interferon-a 2a gene was obtained from human leukocytes. The rHuIFN-a 2a is purified by proprietary chromatographic techniques.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.