Thermo Fisher Scientific JMJD1A Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50

-

Immunocytochemistry (ICC/IF)

1:500-1:2,000

-

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide made to an internal region of the human JMJD1A protein (within residues 250-400). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:JMJD1A,uniProtId:Q9Y4C1-1,ncbiNodeId:9606,antigenRange:250-400,antigenLength:1321,antigenImageFileName:PA5-23066_JMJD1A_Q9Y4C1-1_Rabbit.svg,antigenImageFileNamePDP:PA5-23066_JMJD1A_Q9Y4C1-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.53 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 30% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_11152364

Product Specific Information

The target sequence has 91% sequence homology with rat.

Target Information

JMJD1A is a histone demethylase that specifically demethylates Lys-9 of histone H3, thereby playing a central role in histone code. It preferentially demethylates mono- and dimethylated H3 Lys-9 residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 Lys-9. Demethylation of Lys residue generates formaldehyde and succinate. JMJD1A is involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 Lys-9 demethylation and transcriptional activation. It`s also Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.