Thermo Fisher Scientific JMJD3 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 0.2–1 µg/mL

Product Specifications

Product Specific Information

• Immunogen sequence:
  HRAVDPPGAR AAREAFALGG LSCAGAWSSC PPHPPPRSAW LPGGRCSASI

• Storage guidance:
  For short term use, store at 2–8°C up to 1 week.
  For long term storage, store at -20°C in small aliquots to prevent freeze–thaw cycles.

• Predicted homology:
  Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 93%; Rabbit: 100%; Rat: 100%

Target Information

Covalent modification of histones plays a critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.