Thermo Fisher Scientific PLK4 Monoclonal Antibody (OTI3B10), TrueMAB
PLK4 단백질을 인식하는 마우스 단클론 항체로, Western blot에 적합합니다. Human PLK4(aa 307-612) 재조합 단백질로부터 유래된 면역원 사용. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용 가능. 연구용으로만 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3B10 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 307–612 of human PLK4 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
PLK4 (Polo-like kinase 4) is a serine/threonine protein kinase localized to centrioles, which are microtubule-based structures in centrosomes. It regulates centriole duplication during the cell cycle. Overexpression of PLK4 induces centriole amplification, while reduced gene dosage increases mitotic errors and cancer risk.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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