Thermo Fisher Scientific AFP Monoclonal Antibody (C2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

1:100

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

C2

Immunogen

alpha fetoprotein isolated from serum of hepatoma patients if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:AFP,uniProtId:P02771-1,ncbiNodeId:9606,antigenRange:1-609,antigenLength:609,antigenImageFileName:MA1-35436_AFP_P02771-1_House_mouse.svg,antigenImageFileNamePDP:MA1-35436_AFP_P02771-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

100 µg/mL

Purification

purified

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1070182

Product Specific Information

C2 has been characterized in the ISOBM TD-2 workshop and assigned by K. Nustad to group E of a cluster of 6 major epitopes of human alpha fetoprotein. Human alpha fetoprotein is an oncofetal protein of 70 kDa. It is expressed in fetal liver and is normally absent in health adult tissues. It is positive on all yolk sac tumors, on some other germ cell tumors and on hepatocellular carcinomas.

Target Information

AFP (Alpha Fetoprotein) is a major plasma protein produced by the yolk sac, intestinal tract and the liver during fetal life. AFP expression in adults is often associated with hepatoma or teratoma. However, hereditary persistence of AFP may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and AFP and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. AFP is found in monomeric, dimeric, and trimeric forms. AFP can also bind to copper ions, nickel ions, fatty acids and bilirubin. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. High level of serum AFP has been identified in patients with hepatocellular carcinomas (HCC), teratoblastoma, colorectal cancer, pancreatic cancer, and germ cell neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.