Thermo Fisher Scientific PSD-95 Polyclonal Antibody
PSD-95 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot, IHC, ICC 등 다양한 응용에 적합. 인간, 마우스, 랫트 반응성. 항원 친화 크로마토그래피로 정제되었으며, 고농도 액상 형태로 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse PSD95 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.24 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792886 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (100%), Rat (100%), Bovine (100%).
- Positive Control: mouse eye, mouse brain, mouse fetal brain, rat brain.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Post Synaptic Density 95 kDa (PSD-95), also known as synapse associated protein 90 kDa (SAP90), is a membrane-associated protein found in the postsynaptic density of forebrain neurons and certain presynaptic structures in the cerebellum.
PSD-95 contains three PDZ domains, an SH3 domain, and a Guanylate Kinase (GuK) domain. It is involved in clustering proteins such as NMDA receptors and Shaker-type potassium channels at the synaptic membrane in CNS neurons.
There are two main interaction modes between PSD-95 and other proteins:
- NMDA receptors and Shaker-type potassium channels share a C-terminal T/SXV motif that binds to PSD-95 PDZ domains.
- Neuronal nitric oxide synthase (nNOS), lacking the T/SXV motif but containing a PDZ domain, interacts with PSD-95 via PDZ-PDZ association.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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