Thermo Fisher Scientific Apg12 Polyclonal Antibody
Apg12 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC, ELISA 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. Protein A 정제, 고농도(1 mg/mL) 액상 형태로 안정적 보관 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human Apg12, amino acids 101–187 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
ATG12, a member of the autophagy protein family, forms a conjugate with ATG5 that exhibits ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also interacts with innate immune response proteins such as RIG-I and VISA (IPS-1), inhibiting type I interferon production and facilitating viral replication in host cells. ATG12 interacts with ATG10 in human embryonic kidney cells in the presence of ATG7, and at least two isoforms of ATG12 are known to exist.
Autophagy, the process of bulk degradation of cellular proteins through the autophagosomic-lysosomal pathway, is crucial for normal growth control and may be defective in tumor cells. It helps preserve cellular nutrients under starvation and regulates cytosolic component turnover. This process is negatively regulated by TOR (Target of Rapamycin) through phosphorylation of autophagy protein APG1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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