Thermo Fisher Scientific TCR gamma/delta Monoclonal Antibody (eBioGL3 (GL-3, GL3)), Brilliant Violet 650, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.125 µg/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• Biotin
• eFluor 450
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 645
• Super Bright 780
• Request custom conjugation

Product Specific Information

The GL3 monoclonal antibody reacts with the gamma delta T-cell receptor (TCR) complex on all γδ TCR-bearing T cells, but not with αβ TCR. γδ TCRs are found in thymus, epidermis, intestinal epithelium, peritoneal cavity, and lymphoid tissues.

• Fc Block Recommendation: Pre-stain cells with CD16/CD32 Monoclonal Antibody (Product #14-0161-82) to prevent non-specific Fc-mediated binding.
• Applications Reported: Flow cytometric analysis.
• Applications Tested: Tested by flow cytometry on mouse lymph node cells at ≤0.125 µg/test (100 µL final volume; 10⁵–10⁸ cells/test). Optimize titration for best results.

Fluorochrome Information:
Brilliant Violet™ 650 (BV650) emits at 649 nm and is suitable for cytometers with a violet (405 nm) laser. Ensure instrument compatibility.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product #SB-4401-42) or Brilliant Stain Buffer™ (Product #00-4409-75) to minimize non-specific interactions.

Light Sensitivity:
Protect from light to prevent photo-induced oxidation.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product #00-8222-49)
• 1-step Fix/Lyse Solution (Product #00-5333-54)

Compatibility:
BV650 is not compatible with methanol-based fixation.

Excitation / Emission / Laser:
407 nm / 649 nm / Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

TCR gamma/delta (T-cell receptor γδ) are specialized T-cells involved in immune recognition. They help discriminate self from non-self peptides presented by MHC class II molecules, critical for adaptive immunity.
Mutations or autoantibodies targeting TCRs have been linked to autoimmune diseases and tumor recognition.
γδ T cells are primarily epithelial, showing limited TCR diversity but distinct antigen recognition, with known antitumor and immunoregulatory roles.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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