Thermo Fisher Scientific KDM5B Monoclonal Antibody (1G10)
KDM5B 단백질을 인식하는 Mouse monoclonal antibody (Clone 1G10). Western blot, IHC, ICC, ELISA에 사용 가능. Human 시료에 반응하며, Affinity chromatography로 정제된 액상 제품. -20°C 보관, 방부제 무첨가.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC-P) | 1 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| ELISA | 0.03 ng/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1G10 |
| Immunogen | KDM5B (NP_006609, 231–319 a.a.) partial recombinant protein with GST tag (GST tag MW: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Protein Sequence:
AKRMRAEAMN IKIEPEETTE ARTHNLRRRM GCPTPKCENE KEMKSSIKQE PIERKDYIVE NEKEKPKSRS KKATNAVDLY VCLLCGSGN
Target Information
KDM5B is a histone demethylase that specifically demethylates Lys-4 of histone H3, playing a central role in histone code regulation. It does not demethylate H3 Lys-9 or Lys-27. KDM5B acts as a transcriptional corepressor for FOXG1B and PAX9, promoting proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. Conversely, it may act as a tumor suppressor in melanoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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