Thermo Fisher Scientific MBD4 Polyclonal Antibody
Rabbit polyclonal antibody recognizing human MBD4 protein. Validated for IHC and IHC-P applications with dilution range 1:100–1:500. Supplied in liquid form, 0.25 mg/mL, purified by antigen affinity chromatography. Recommended storage at 4°C, for resea...
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1 and 50 of human methyl-CpG binding domain protein 4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Based on 100% sequence identity, this antibody is predicted to react with Gorilla and Chimpanzee
Target Information
DNA methylation, the addition of methyl groups to cytosine bases in CpG dinucleotides, is essential for proper development and gene regulation. The demethylation enzyme associated with the methyl-CpG-binding domain (MBD) can revert methylated cytosine to cytosine by cleaving the methyl group as methanol.
MeCP2 and MBD1 repress transcription by binding to methylated DNA, while MBD2 and MBD4 (also known as MED1) colocalize with heavily methylated satellite DNA and mediate methylation signaling. MBD3 does not bind methylated DNA.
MBD1–4 are expressed in somatic tissues, but MBD1 and MBD2 expression is reduced or absent in embryonic stem cells. MBD4 shares homology with bacterial base excision repair DNA N-glycosylases/lyases and can be mutated in microsatellite unstable tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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