Thermo Fisher Scientific E2F1 Polyclonal Antibody
E2F1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, ICC/IF, IP에 사용 가능. 인간 반응성, 고순도 항원 친화 크로마토그래피 정제. 세포주 및 전사인자 연구용으로 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 43–217 of Human E2F1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2544841 |
Product Specific Information
- Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, NIH-3T3, E2F1-transfected 293T
- Predicted reactivity: Mouse (96%), Rat (97%), Chicken (86%), Bovine (96%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of the cell cycle and the action of tumor suppressor proteins. It is also a target of transforming proteins of small DNA tumor viruses.
E2F proteins contain several evolutionarily conserved domains, including:
- DNA binding domain
- Dimerization domain (interaction with DP transcription factors)
- Transactivation domain enriched in acidic amino acids
- Tumor suppressor protein association domain (within transactivation domain)
E2F1, along with E2F2 and E2F3, has an additional cyclin-binding domain. It binds preferentially to retinoblastoma protein pRB in a cell-cycle-dependent manner and can mediate both cell proliferation and p53-dependent/independent apoptosis.
⚠ WARNING: This product can expose you to chemicals including mercury, known to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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