Takara HRV 3C Protease for fusion tag cleavage from recombinant proteins

Overview

• Active at 4°C, allowing the cleavage reaction to proceed under conditions that do not affect the activity or stability of the recombinant target protein
• 6xHN tag allows removal of the 3C protease from the reaction using IMAC

More Information

Applications

• Cleaving fusion tags (e.g., His, TF, ProS2, GST) from recombinant fusion proteins

Components

• 500 Units HRV 3C Protease (1 U/µl)
• 10 µl Cleavage Control Fusion Protein (1 µg/µl)
• 10 ml 10X HRV 3C Cleavage Buffer

Activity definition

1 unit (U) is defined as the amount of enzyme that cleaves at least 95% of the Cleavage Control Fusion Protein (100 µg in 1X HRV 3C Cleavage Buffer) in 16 hours at 4°C.

Storage

–20°C

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.