Thermo Fisher Scientific MANEA Polyclonal Antibody
Human MANEA 단백질을 인식하는 Rabbit Polyclonal Antibody로, IHC(P)에서 1:20~1:50 희석으로 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS/glycerol buffer에 보관. 연구용으로만 사용.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P)): 1:20–1:50
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human MANEA. Recombinant protein control fragment (Product # RP-90480) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2643727 |
Product Specific Information
Immunogen sequence:
GVLALSWYPP DVNDENGEPT DNLVPTILDK AHKYNLKVTF HIEPYSNRDD QNMYKNVKYI IDKYGNHPAF YRYKTKTGNA LPMFYVYDSY ITKPEKWANL LTTSGSRSIR NSPYDGLFIA LLVEEKHKYD ILQSGFDGIY T
- Highest antigen sequence identity to orthologs:
- Mouse: 87%
- Rat: 88%
Target Information
N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex.
This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi.
MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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