Thermo Fisher Scientific MANEA Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:50

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human MANEA. Recombinant protein control fragment (Product #RP-90480). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MANEA,uniProtId:Q5SRI9-1,ncbiNodeId:9606,antigenRange:182-322,antigenLength:462,antigenImageFileName:PA5-52784_MANEA_Q5SRI9-1_Rabbit.svg,antigenImageFileNamePDP:PA5-52784_MANEA_Q5SRI9-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2643727

Product Specific Information

Immunogen sequence: GVLALSWYPP DVNDENGEPT DNLVPTILDK AHKYNLKVTF HIEPYSNRDD QNMYKNVKYI IDKYGNHPAF YRYKTKTGNA LPMFYVYDSY ITKPEKWANL LTTSGSRSIR NSPYDGLFIA LLVEEKHKYD ILQSGFDGIY T

Highest antigen sequence identity to the following orthologs: Mouse - 87%, Rat - 88%.

Target Information

N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.