Thermo Fisher Scientific GFI1 Polyclonal Antibody
GFI1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, ICC/IF, Flow cytometry에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 인체 반응성이 확인됨. 연구용으로만 사용.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| Flow Cytometry (Flow) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human GFI1. The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.49 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2735834 |
Product Specific Information
- Positive Control: THP-1, HL-60
- Predicted Reactivity: Mouse (80%), Rat (81%), Dog (87%), Cat (89%), Rhesus Monkey (98%), Bovine (88%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
GFI1 is a nuclear zinc finger (ZF) transcriptional repressor that plays an important role in hematopoiesis and inner ear development, and has been implicated in lymphomagenesis.
The Gfi1 protein expression is regulated by the ubiquitin-proteasome system. It is reported that ubiquitin ligase Triad1 interacts with the DNA-binding domain of Gfi-1.
Gfi-1 represses transcription by directly binding to the consensus DNA sequence in the promoters of its target genes.
Deficiency of Gfi-1 causes neutropenia and an accumulation of granulomonocytic precursor cells that is reminiscent of a myelodysplastic syndrome in mice.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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