Thermo Fisher Scientific MED9 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.5 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL

-

Immunocytochemistry (ICC/IF)

2 µg/mL

-

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells

-

Product Specifications

Species Reactivity

Rat, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived human MED9 recombinant protein (Position: A55-E146). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MED9,uniProtId:Q9NWA0-1,ncbiNodeId:9606,antigenRange:55-146,antigenLength:146,antigenImageFileName:PA5-79661_MED9_Q9NWA0-1_Rabbit.svg,antigenImageFileNamePDP:PA5-79661_MED9_Q9NWA0-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

0.05mg sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2746776

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Target Information

In mammalian cells, transcription is regulated in part by high molecular weight co-activating complexes that mediate signals between transcriptional activators and RNA polymerase II (Pol II). The mediator complex is one such multi-protein structure that functions as a bridge between regulatory proteins and Pol II, thereby regulating Pol II-dependent transcription. Med9 (mediator complex subunit 9), also known as MED25, is a 146 amino acid nuclear protein and component of the mediator complex. Med9 directly interacts with Med4 and is encoded by a gene that maps to human chromosome 17p11.2. Chromosome 17 comprises over 2.5% of the human genome, encodes over 1,200 genes and is associated with two key tumor suppressor genes, namely, p53 and BRCA1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.