Thermo Fisher Scientific Cytokeratin 20 Polyclonal Antibody
Cytokeratin 20 단백질을 검출하기 위한 Rabbit Polyclonal 항체로, Western blot, IHC, ICC, Flow Cytometry에 적합합니다. Human, Mouse, Rat 시료에 반응하며, 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within Human Cytokeratin 20 (aa 375–424) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS (pH 7.4) with 50% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long term, store at –20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2901078 |
Product Specific Information
Positive controls:
Human small intestine tissue lysates, mouse colon tissue lysates, SW480, human colon tissue, human small intestine tissue, mouse colon tissue, mouse small intestine tissue, JAR.
Target Information
The protein encoded by this gene is a member of the keratin family. Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins.
Type I cytokeratins consist of acidic proteins arranged in pairs of heterotypic keratin chains. Cytokeratin 20 is a major cellular protein of mature enterocytes and goblet cells, specifically expressed in the gastric and intestinal mucosa.
Type I cytokeratin genes are clustered in the chromosome region 17q12–q21.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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