Thermo Fisher Scientific CD7 Monoclonal Antibody (eBio124-1D1 (124-1D1)), Biotin, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 737
• Brilliant Violet 650
• eFluor 450
• FITC
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 700
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

Description:
The eBio124-1D1 monoclonal antibody reacts with human CD7, also known as gp40 and Leu9. CD7 is a 40 kDa receptor and a member of the immunoglobulin gene superfamily. The N-terminal amino acid sequence (aa1–107) is highly homologous to Ig kappa light chain sequence, while the carboxyl-terminal region is proline-rich and forms a stalk projecting the Ig domain.

CD7 is expressed on most immature and mature T lymphocytes and T cell leukemias, as well as on natural killer cells, a small subset of normal B cells, and malignant B cells. Cross-linking of surface CD7 modulates T cell and NK cell activity, influencing calcium flux, adhesion molecule expression, cytokine secretion, and proliferation. CD7 directly associates with phosphoinositol 3′-kinase, leading to D-3 phosphoinositide production and tyrosine phosphorylation upon ligation.

A clonogenic subset of human CD34(+) CD38(-) cord blood cells expressing CD45RA, HLA-DR, and high levels of CD7 has been identified as lymphoid progenitors capable of generating B cells, NK cells, and dendritic cells, but not myeloid or erythroid cells. CD7(+) phenotype distinguishes primitive human lymphoid progenitors from pluripotent stem cells.

Additionally, CD7 may cooperate with CD28 in Treg function; mice lacking both CD28 and CD7 exhibit reduced Treg populations and suppressive activity.

Applications Reported:
This antibody is reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of normal human peripheral blood cells. Recommended usage ≤1 µg per test (100 µL final volume). Cell numbers may range from 10⁵ to 10⁸ cells/test. Optimal titration is recommended.

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

CD7 (gp40, Leu9) is a 40 kDa member of the immunoglobulin gene superfamily. The N-terminal region (aa1–107) is homologous to Ig kappa-L chains, while the carboxy-terminal domain is proline-rich. CD7 is expressed on most immature and mature T-lymphocytes, T cell leukemias, NK cells, and a subset of B cells. Cross-linking of CD7 modulates immune cell activity through calcium flux, adhesion molecule expression, cytokine secretion, and proliferation. CD7 associates with phosphoinositol 3′-kinase, and ligation induces D-3 phosphoinositide formation and tyrosine phosphorylation. CD7 expression is a key marker in leukemia diagnostics.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.