Thermo Fisher Scientific Cortactin Recombinant Rabbit Monoclonal Antibody (ARC1613)
Thermo Fisher의 Cortactin Recombinant Rabbit Monoclonal Antibody (ARC1613)은 인간, 마우스, 랫트에 반응하며 WB, IHC, ICC/IF, ELISA, IP 등에 적합합니다. HEK293 세포에서 발현된 재조합 항체로 높은 특이성과 안정성을 제공합니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific Cortactin Recombinant Rabbit Monoclonal Antibody (ARC1613)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:6,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:800 |
| Immunocytochemistry (ICC/IF) | 1:200–1:800 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 400 µg–600 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC1613 |
| Immunogen | A synthetic peptide corresponding to amino acids 1–100 of human Cortactin (Q14247) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849698 |
Product Specific Information
Immunogen sequence:
MWKASAGHAV SIAQDDAGAD DWETDPDFVN DVSEKEQRWG AKTVQGSGHQ EHINIHKLRE NVFQEHQTLK EKELETGPKA SHGYGGKFGV EQDRMDKSAV
Target Information
Cortactin (Cttn) is a ubiquitous actin-binding protein originally identified as a substrate for Src. It contributes to actin cytoskeleton organization and cell shape, and is involved in lamellipodia formation and cell migration. Cortactin accumulates in actin-enriched peripheral structures, facilitating actin network formation. It contains four major domains: N-terminal acidic (NTA), tandem repeats, proline-rich, and SH3 domains. NTA associates with the Arp2/3 and WASP complexes at F-actin branches. Cortactin promotes cell motility and invasion, playing a critical role in invadopodia formation involved in ECM degradation by cancer cells. It is phosphorylated by Src family kinases at Y421, Y466, Y482 and by Erk family kinases at S405 and S418.
For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.
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