Thermo Fisher Scientific CD33 Monoclonal Antibody (WM-53 (WM53)), NovaFluor Yellow 660, eBioscience

Thermo Fisher Scientific CD33 Monoclonal Antibody (WM-53 (WM53)), NovaFluor Yellow 660, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.8 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 496
• Brilliant UV 661
• Brilliant Violet 605
• Brilliant Violet 711
• FITC
• NovaFluor Blue 690
• NovaFluor Red 710
• NovaFluor Yellow 570
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

Description:
WM-53 monoclonal antibody reacts with human CD33 (GP67, P67), a 67 kDa type I transmembrane glycoprotein of the Siglec family. CD33 is expressed in the hematopoietic compartment, including monocytes, activated T cells, granulocytes, myeloid progenitors, and mast cells.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and validated for flow cytometry using normal human lysed whole blood. Recommended usage: 5 µL (0.8 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining with NovaFluor conjugates.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, incorporating novel nucleic acid dye structures for engineered fluorescence with minimized spillover and spread.

Excitation: 552 nm
Emission: 663 nm
Laser: 561 nm (Yellow)

Target Information

CD33 is a transmembrane protein of the Siglec family containing ITIM motifs that recruit SHP-1 and SHP-2 phosphatases. It is expressed on myelomonocytic lineage cells, binds sialic acid residues on N- and O-glycans, and is a therapeutic target for acute myeloid leukemia. CD33 is found on granulocyte and macrophage precursors but not on pluripotent stem cells, making it a useful marker for distinguishing myelogenous leukemia cells. Diseases associated with CD33 dysfunction include gallbladder lymphoma and extracutaneous mastocytoma.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without authorization.

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