Thermo Fisher Scientific CRY1 Monoclonal Antibody (OTI2F5), TrueMAB

Applications

• Western Blot (WB): Tested dilution 1:2,000

Product Specifications

Target Information

Various biochemical, physiological, and behavioral processes display circadian rhythms controlled by an internal biological clock. The central gears driving this clock appear to be composed of an autoregulatory transcription/post-translation-based feedback loop.

Cryptochrome 1 (CRY1) and CRY2 are DNA-binding flavoproteins that bear some homology to blue-light receptors and photolyases. In Drosophila, CRY acts as a photoreceptor for the circadian clock, binding to the clock component TIM in a light-dependent fashion and blocking its function.

Mammalian CRY1 and CRY2 function via light-independent interactions with circadian genes CLOCK and BMAL1, as well as with PER1, PER2, and TIM. They act as light-independent components of the circadian clock and likely regulate Per1 transcriptional cycling through interactions with both activators and feedback inhibitors.

Mutant mice lacking Cry1 or Cry2 display accelerated or delayed periodicity of locomotor activity, respectively. The combination of both proteins is essential for synchronizing the organism to circadian phases. A critical balance between Cry1 and Cry2 is required for proper clock function; in complete darkness, double-mutant mice exhibit instantaneous arrhythmicity, indicating absence of an internal circadian clock.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.