Thermo Fisher Scientific Phospho-p53 (Ser46) Monoclonal Antibody (GT1018)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Positive Control: 293T, 293T (0.5 µM doxorubicin treatment for 24 hr)
• Store product as a concentrated solution.
• Centrifuge briefly prior to opening the vial.

Target Information

The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nutrient deprivation. p53 up-regulates stress response genes such as p21waf1, leading to G1 or G2/M arrest.

The structure of p53 includes:

• N-terminal transactivation domain
• Central DNA-binding domain
• Oligomerisation domain
• C-terminal regulatory domain

Phosphorylation at Ser15 is important for p53 activation and stabilization, while phosphorylation at Ser392 is associated with tumor formation. p53 plays a key role in preventing proliferation of damaged cells and functions as an anticancer factor. It is also linked to aging, oxidative stress, and cognitive processes such as long-term potentiation (LTP).

Mutations in the TP53 gene are common in human cancers and can lead to loss of tumor suppressor activity. Germline mutations are also associated with cancer-prone syndromes such as Li-Fraumeni syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.