Thermo Fisher Scientific NUP50 Polyclonal Antibody
NUP50 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, IP 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 인체 시료에 반응합니다. 액상 형태로 4°C에서 보관하며, 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 225 and 275 of human nucleoporin 50kDa (NUP50) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
The nuclear pore complex is a large structure spanning the nuclear envelope that regulates the transport of macromolecules between the nucleus and cytoplasm.
Nucleoporins are the main components of this complex in eukaryotic cells.
The protein encoded by the NUP50 gene is an FG-repeat containing nucleoporin that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import.
Pseudogenes are found on chromosomes 5, 6, and 14, and two transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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