Thermo Fisher Scientific Calbindin D28K Polyclonal Antibody
Calbindin D28K 단백질을 인식하는 Thermo Fisher Scientific의 고트 폴리클로날 항체로, WB 및 IHC에 적합합니다. 인간과 랫트 반응성이 있으며, 고순도 액상 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.3 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–7 µg/mL |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 5–7 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide with sequence C-KTFVDQYGQRDDGK (aa 59–72) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2933605 |
Product Specific Information
- Immunizing peptide overlaps EF hand 2, but not any calcium binding site.
- Tested in Peptide ELISA: antibody detection limit dilution 1:128,000.
Target Information
Calbindin-D-28K (also termed vitamin D-dependent calcium-binding protein, or cholecalcin) is a highly conserved 28 kDa calcium-binding protein with broad tissue distribution.
It belongs to a family of low molecular weight calcium-binding proteins (CaBPs) including calmodulin, S-100, parvalbumin, and troponin C.
These CaBPs share homologous EF-hand type structures for Ca²⁺ binding.
Calbindin-D-28K is predominantly found in subpopulations of central and peripheral nervous system neurons, and in epithelial cells involved in Ca²⁺ transport such as distal tubular and cortical collecting tubules of the kidney, and enteric neuroendocrine cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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