Thermo Fisher Scientific Connexin 30.2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2-4 µg/mL

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the c-term region of the mouse connexin 30.2 protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Connexin 30.2,uniProtId:Q91YD1-1,ncbiNodeId:10090,antigenRange:278,antigenLength:278,antigenImageFileName:40-7400_Connexin_30.2_Q91YD1-1_Rabbit.svg,antigenImageFileNamePDP:40-7400_Connexin_30.2_Q91YD1-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533480

Product Specific Information

40-7400 was used in the western blot analysis to successfully detect Connexin 30.2 in HeLa cells transfected with Cx30.2 and in immunofluorescence (IHC) analysis to successfully detect Connexin 30.2 in mouse heart sections.

Target Information

Gap junctions are responsible for transport of ions and metabolites between adjacent cells. These structures are made of two hemichannels, each formed by six connexin molecules. Connexin 30, coded by Cjb6 gene, is a member of the connexin family and is highly expressed in brain and skin. Immunohistochemically connexin 30 was localized in astrocytes, at gap junctions between these cells and on the astrocyte side of gap junctions between astrocytes and oligodendrocytes. Co-localization with connexin 43 was also observed in this study. Cytoskeleton, especially actin filaments, are important components in the processes of assembly, trafficking and stabilization of connexin 30 gap junctions. Knock-out mice studies have also demonstrated that connexin 30 deficiency impairs renal tubular ATP release and pressure natriuresis highlighting the importance of this molecule in kidney. Further, in vitro studies have also demonstrated that connexin 30 overexpression enhances cell proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.