Thermo Fisher Scientific Goat anti-Mouse IgG2a Secondary Antibody, Biotin

Applications and Tested Dilution

• Western Blot (WB): 1:5,000–1:50,000
• Immunocytochemistry (ICC/IF): 1:10,000

Product Specifications

Available Conjugate Formats

• Alexa Fluor Plus 488
• Alexa Fluor Plus 555
• Alexa Fluor Plus 647
• Alexa Fluor Plus 680
• Alexa Fluor Plus 800
• HRP
• PE-Cyanine5.5
• Custom conjugation available upon request

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins. They are useful for detection, sorting, or purification of the specified target.
Secondary antibodies provide versatility across multiple detection systems (e.g., HRP, AP, fluorescence) and enhance sensitivity via signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, they are generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.