
Thermo Fisher Scientific Human TNF-RII (soluble) Recombinant Protein
인간 TNF-RII 재조합 단백질로 TNF-α 관련 기능 연구에 적합. E. coli 발현 시스템에서 생산되며 ≥98% 순도 확보. 세포 독성 억제 기능 검증 완료. ELISA, WB, IHC 등 다양한 분석에 활용 가능.
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Thermo Fisher Scientific Human TNF-RII (soluble) Recombinant Protein
Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | Human TNF-RII recombinant protein contains 174 amino acids |
| Molecular Weight | 18.9 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | ≥98% by SDS-PAGE and HPLC |
| Endotoxin Concentration | <0.1 ng/µg |
| Activity | ED50 = 0.125 µg/mL; determined by dose-dependent inhibition of TNF-α (0.25 ng/mL) mediated cytotoxicity in mouse L-929 cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | 10 mM sodium phosphate, pH 8, with 50 mM NaCl |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice (domestic); Wet or Dry ice (international) |
Product Specific Information
- Reconstitution:
Briefly centrifuge vial before opening. Reconstitute sTNF-RII in sterile distilled water (0.1–1.0 mg/mL). Further dilutions should be made in aqueous buffers with carrier protein (0.1–1.0% BSA). Do not vortex. Store reconstituted solution at 2–8°C up to 1 week. For long-term storage, dilute in buffer with 0.1% BSA and store aliquots at -20°C to -80°C. - Storage:
Lyophilized protein is stable at room temperature for 1 month; store at -20°C for long-term. Working aliquots with carrier protein are stable ≥3 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
Target Information
TNFR2 (tumor necrosis factor receptor superfamily member 1B) is a receptor with high affinity for TNFSF2/TNF-α and lower affinity for homotrimeric TNFSF1/lymphotoxin-α. The TRAF1/TRAF2 complex recruits apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. TNFR2 mediates most metabolic effects of TNF-α.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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