Thermo Fisher Scientific Human TNF-RI (soluble) Recombinant Protein
인간 TNF-RI(용해형) 재조합 단백질로, TNF-α 매개 세포독성 억제 기능을 평가하는 연구용 시약입니다. 고순도(>98%) 및 낮은 엔도톡신 수준(<0.1 ng/µg)을 보유하며, E. coli 발현 시스템에서 생산되었습니다. ELISA, WB, IHC, 기능 분석 등 다양한 생명공학 연구에 적합합니다.
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Thermo Fisher Scientific Human TNF-RI (soluble) Recombinant Protein
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | Assay-dependent |
| ELISA | Assay-dependent |
| Functional Assay | Assay-dependent |
| Control (Ctrl) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | Human TNF-RI recombinant protein contains 162 amino acids |
| Molecular Weight | 18.3 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >98% by SDS-PAGE and HPLC |
| Endotoxin Concentration | <0.1 ng/µg |
| Activity | ED50 = 0.05 µg/mL; determined by the dose-dependent inhibition of TNF-alpha (0.25 ng/mL) mediated cytotoxicity in mouse L-929 cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | 5 mM Tris, pH 8 |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice (domestic); Wet or Dry ice (international) |
Product Specific Information
- Reconstitution: Briefly centrifuge the vial before opening. Reconstitute sTNF-RI in 5 mM Tris, pH 8.0, to a concentration of 0.1–1.0 mg/mL. Further dilutions should be made in aqueous buffers supplemented with carrier protein (0.1–1.0% BSA).
- Storage: Lyophilized preparation is stable at 2–8°C, but should be kept at -20°C for long-term storage, preferably desiccated. Upon reconstitution, stable for up to one week at 2–8°C. For maximal stability, store aliquots at -20°C. Avoid repeated freeze/thaw cycles.
Target Information
TNFR1 (Tumor necrosis factor receptor 1) is a member of the TNF superfamily and a major receptor for TNF-α. It mediates cytokine signaling, positively regulates the NF-κB pathway and angiogenesis, and negatively regulates gene expression. The extracellular domain of TNFR1 can be released into circulation as soluble TNFR1 (sTNFR1). In humans, the TNFR1 gene is located on chromosome 12. Interaction partners include BAG4/SODD, TRADD, and TRAF2, which regulate TNFR1-mediated signal transduction. Mutations in TNFR1 extracellular domains are associated with autosomal dominant periodic fever syndrome due to impaired receptor clearance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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