Thermo Fisher Scientific TID1 Monoclonal Antibody (RS13)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

2 µg/mL

-

Immunoprecipitation (IP)

2 µg/mL

-

Product Specifications

Published species

Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

RS13

Immunogen

Recombinant hTid-1 protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10984230

Product Specific Information

MA5-12267 targets TID-1 in IP, IP, and WB applications and shows reactivity with Human, mouse, and Rat samples.

The MA5-12267 immunogen is recombinant hTid-1 protein.

Target Information

TID1 is a human homolog of the Drosophila tumor suppressor lethal (2) tumorous imaginal discs, l(2)tid and encodes two mitochondrial matrix localized splice variants of human Tid-1 designated hTid-1S and hTid-1L. These proteins are the conserved members of the DnaJ family of proteins which act as cochaperones for mitochondrial Hsp70. They contain a conserved tetrahedrical J domain which binds to Hsp70 chaperones and activates their ATPase activity. Expression of hTid-1L increases apoptosis induced by DNA damaging agents as mitomycin-C and TNF-a. A J-domain mutant of hTid-1L can dominantly suppress apoptosis and in sharp contrast the J-domain mutant of hTid-1S increases apoptosis. Expression of hTid-1S and hTid-1L affects cytochrome c release from the mitochondria and caspase 3 activation, while activation of caspase 8 is unaffected. It is strongly suggested that these two splice variants exert their anti- and pro- apoptotic effects through discrete substrates and activities. Hence the relative abundance of these proteins or their substrates may allow the mitochondria to dampen or enhance the apoptotic signals.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.