Norgen Adenovirus Purification Kit

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카탈로그번호: 67600
브랜드: Norgen
카테고리: Virus Reagents
판매단위: pk

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Norgen · Norgen Adenovirus Purification Kit

Norgen Adenovirus Purification Kit

SKU: 67600
For concentrating and purifying adenoviral vectors from cell lysate and cell culture media.

Features and Benefits

Adenovirus purification from any input – cell fraction or media fraction
Rapid purification within 2 to 4.5 hours
No specialized equipment needed (ultracentrifuge not required)
Purify adenovirus cell culture supernatant from 1 mL to 33.5 mL input per prep
Purify adenovirus cell pellet from 1 mL of input per prep
Up to 25X sample concentration
Purification based on spin column chromatography using Norgen’s proprietary resin separation matrix

Product Description

Adenoviral vectors are useful tools for both in vitro and in vivo gene transfer, and oncolytic viruses based on adenovirus are highly promising for cancer treatment.
Norgen’s Adenovirus Purification Kit provides a fast and simple procedure for concentrating and purifying adenoviral vectors from cell lysate and cell culture media.
Purification is based on spin column chromatography using Norgen’s proprietary resin as an ion exchanger. Contaminating cellular debris is largely removed via centrifugation, while contaminating DNA and RNA are reduced using enzymatic digestion.
Adenoviral vectors purified in this manner are highly active for use in transduction experiments.

This kit contains sufficient materials for 15 preparations (33.5 mL per prep of supernatant or a total of 500 mL of supernatant input). Approximately 1 mL of cell pellet can be purified per prep, up to a maximum of 15 mL of cell pellet for the entire kit.

Kit Specifications

Parameter	Description
Resin Binding Capacity (total per kit)	≥ 3×10¹¹ adenovirus particles (qPCR) / ≥ 3×10⁸ transducing units (transduction assay)
Input Type	Cells, media
Input Volume (Supernatant)	1 – 33.5 mL per prep (500 mL total)
Input Volume (Cell Pellet)	1 mL per prep (15 mL total)
Minimum Elution Volume	1.3 mL per prep
Time to Complete Purification	2.5 – 4.5 hours (1 hour hands-on)
In vivo Transduction	Yes

Storage Conditions and Product Stability

HL-SAN Nuclease: Store at -20°C
Elution Buffer P: Store tightly capped at 4°C
All other solutions: Store at room temperature, tightly sealed
Shelf life: Stable for 2 years after shipment

Kit Components

Component	Cat. 67600 (15 preps)
Lysis Buffer S	5.5 mL
HL-SAN Nuclease	102 µL
Binding Buffer A	20 mL
Purification Solution C	60 mL
Purification Solution D	130 mL
Wash Solution C	2 × 130 mL
Slurry E	12.5 mL
Elution Buffer P (store at 4°C)	66 mL
Protein Neutralizer	4 mL
Elution Tubes (1.7 mL)	50
Product Insert	1

Supporting Data

Figure 1. HeLa cells were infected with the eluted adenovirus vector expressing the beta-galactosidase reporter gene. The cells were fixed and stained 48 hours post infection. The dark staining indicates cellular transduction by the adenoviral vector and expression of the reporter gene.

Figure 2. Total adenoviral vector eluted for 1, 3, or 15 mL of supernatant containing adenovirus.
An increase in titer is observed as the volume of adenovirus-containing supernatant increases, demonstrating effective purification at different volumes.
이미지 내 텍스트:

Y축: Ad copy #
X축: Input (mL supernatant)
R² = 0.98

Figure 3. Adenovirus vector was eluted from the kit from various volumes of supernatant: 1 mL, 3 mL, and 15 mL.
Three separate elutions (500 µL each) were performed with Elution Buffer P.

이미지 내 데이터 표:

	1 mL	3 mL	15 mL
Elution 1	6.81E+08	1.50E+09	4.48E+09
Elution 2	1.26E+09	2.92E+09	6.41E+09
Elution 3	1.62E+09	3.39E+09	8.21E+09

Documentation

AAV Purification Kit

제품 이미지

Figure 1 - HeLa cells infected with adenovirus vector
Figure 2 - Total adenoviral vector eluted
Figure 3 - Adenovirus vector elution volumes
Related Product - AAV Purification Kit