Thermo Fisher Scientific Importin 7 Polyclonal Antibody
Importin 7 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, WB, IHC, IP 등 다양한 응용에 적합합니다. 인간 및 생쥐 반응성, 항원 친화 크로마토그래피로 정제, 액상 형태이며 4°C에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:200–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunoprecipitation (IP) | 5–15 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 200 and 250 of human Importin 7 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta. Similar to importin-beta, this protein prevents the activation of Ran’s GTPase by RanGAP1 and inhibits nucleotide exchange on RanGTP. It also binds directly to nuclear pore complexes, competing for binding sites with importin-beta and transportin. This protein has a Ran-dependent transport cycle and can cross the nuclear envelope rapidly and bidirectionally. At least four importin beta-like transport receptors—importin beta itself, transportin, RanBP5, and RanBP7—directly bind and import ribosomal proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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