Invitrogen™

TOP10F` Electrocomp™ Kit

TOP10F´ Electrocomp Kit E. coli cells are identical to TOP10 cells, with the addition of an F´ episome. The F´자세히 알아보기

TOP10F´ Electrocomp Kit E. coli cells are identical to TOP10 cells, with the addition of an F´ episome. The F´ episome carries the tetracycline resistance gene and allows isolation of single-stranded DNA (ssDNA) from vectors that have an f1 origin of replication. In addition, the F´ episome carries the lac_Iq repressor for inducible expression from _trc, tac, and lac promoters using IPTG.

TOP10F´ competent cells are transformed via electroporation, thus achieving higher transformation efficiencies than chemically competent cells and may be used in more challenging applications, including cloning a low amount of DNA or cDNA and gDNA library construction. These cells allow stable replication of high copy number plasmids and are provided at a transformation efficiency of >1 x 1010 cfu/ μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation.

As a derivative of TOP10, TOP10F´ cells carry mutations in the methylation-dependent restriction system (mcr_A, _mcr_BC, and _mrr) allowing one to clone both prokaryotic and eukaryotic genomic DNA, as well as efficient plasmid rescue from eukaryotic genomes. Similar to other DH strains, the TOP10 strain also has _rec_A1 and _end_A1 mutations to increase insert stability and improve the quality of plasmid DNA prepared from minipreps.

TOP10F´ Electrocomp Kit E. coli cells offer:
• Transformation efficiencies of >1 x 1010 cfu/μg
• Stable F´ episome that allows isolation of ssDNA and carries lac_Iq repressor for inducible expression from _trc, tac, and lac promoters
• _hsd_R for efficient transformation of unmethylated DNA from PCR amplifications
• _mcr_A for efficient transformation of methylated DNA from genomic preparations
• _lac_ZΔM15 for blue/white color screening of recombinant clones on plates containing either X-Gal or Bluo-Gal
• _end_A1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
• _rec_A1 for reduced occurrence of nonspecific recombination in cloned DNA

Note: TOP10F´ cells require IPTG induction for blue-white color screening. A high-voltage electroporation apparatus is also required.

Genotype
F´ [lac_Iq, _Tn_10(TetR)] _mcr_A Δ(_mrr_-_hsd_RMS-_mcr_BC) φ80_lac_ZΔM15 Δ_lac_X74 _rec_A1 _ara_D139 Δ(_ara-leu)7697 _gal_U _gal_K _rps_L(StrR) _end_A1 _nup_G

Find the strains and formats that fit your needs
We offer other DH strains in chemically competent and electrocompetent cell.
Electroporation cuvettes with 0.1 cm, 0.2 cm, and 0.4 cm gaps are available.
The TOP10 strain is available in several MultiShot formats for high throughput applications.
We offer other strains for ssDNA production, such as DH12S.
Explore bacterial growth media formats.

사양

제품 유형Electrocompetent Cells

플라스미드 품질 개선Yes (endA1)

메틸화 DNA 클로닝Yes (mcrA)

형질전환 효율 수준High Efficiency (>1010 cfu/μg)

불안정 DNA 클로닝Not suitable for cloning unstable DNA

블루/화이트 스크리닝Yes (lacZΔM15)

플라스미드May be used for plasmids >20 kb

비메틸화 DNA 준비No

재조합 감소Yes (recA1)

배송 조건Dry Ice

종E. coli (K12)

형식Tube

제품라인Electrocomp™

수량10 x 100 μL

Unit SizeEach