Thermo Fisher Scientific TOP10 Electrocomp Kit, 10 x 100 μL
| 상품 옵션 정보 | ||||||||
|---|---|---|---|---|---|---|---|---|
| 카탈로그 번호 | CAS 번호 | 설명 | 상태 | 단위 | 판매가 | 할인가 | 가격(VAT포함) | 수량 / 장바구니 / 찜 |
| C66411 | - | Thermo Fisher Scientific C66411 TOP10 Electrocomp Kit, 10 x 100 uL Each pk | 재고문의 | pk | 855,000원 | - | 940,500원 | |
| C66455 | - | Thermo Fisher Scientific C66455 TOP10 Electrocomp Kit, 5 x 100 uL Each pk | 재고문의 | pk | 466,000원 | - | 512,600원 | |
다른 상품 둘러보기
Invitrogen™
TOP10 Electrocomp™ Kit
TOP10 Electrocomp E. coli cells are provided at a transformation efficiency of 1 x 1010 cfu/μg supercoiled DNA and are자세히 알아보기
TOP10 Electrocomp E. coli cells are provided at a transformation efficiency of 1 x 1010 cfu/μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation. This strain is used in wide range of applications and allows stable replication of high-copy number plasmids and maintenance of large plasmids.
The TOP10 genotype is similar to the DH10B strain and features:
• _hsd_R for efficient transformation of unmethylated DNA from PCR amplifications
• _mcr_A for efficient transformation of methylated DNA from genomic preparations
• _lac_ZΔM15 for blue/white color screening of recombinant clones
• _end_A1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by Endonuclease I
• _rec_A1 for reduced occurrence of non-specific recombination in cloned DNA
*Notes
*Please see the product manual for reaction scale recommendations. A high-voltage electroporation apparatus is required.
Genotype
F- mcr_A_ Δ(mrr_-hsd_RMS-_mcr_BC) φ80_lac_ZΔM15 Δ_lac_X74 rec_A1_ ara_D139 Δ(ara_-_leu)7697_ gal_U_ gal_K_ rps_L_ end_A1_ nup_G_
Find the strain and format that fits your needs
TOP10 is available in chemically competent and electrocompetent cell formats.
Choose MultiShot competent cells for high-throughput cloning applications.
Email us for more information about custom formats.
사양
제품 유형Electrocompetent Cells
에프에피솜 포함No
플라스미드 품질 개선Yes (endA1)
메틸화 DNA 클로닝Yes (mcrA)
형질전환 효율 수준High Efficiency (>1 x 1010 cfu/μg)
항생제 내성 박테리아Yes (Streptomycin)
불안정 DNA 클로닝Not suitable for cloning unstable DNA
블루/화이트 스크리닝Yes (lacZΔM15)
고처리량 호환성Low
플라스미드May be used for plasmids >20 kb
비메틸화 DNA 준비No
재조합 감소Yes (recA1)
배송 조건Dry Ice
T1 Phage - 저항성(tonA)No
종E. coli (K12)
형식Tube
제품라인Electrocomp™
수량10 x 100 μL
Unit SizeEach
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