Thermo Fisher Scientific STRAP Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

-

Immunocytochemistry (ICC/IF)

1:50-1:100

-

Flow Cytometry (Flow)

1:500-1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein within C-terminal human Unrip from aa 200-350 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:STRAP,uniProtId:Q9Y3F4-1,ncbiNodeId:9606,antigenRange:200-350,antigenLength:350,antigenImageFileName:PA5-119874_STRAP_Q9Y3F4-1_Rabbit.svg,antigenImageFileNamePDP:PA5-119874_STRAP_Q9Y3F4-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.4, with 50% glycerol, 0.2% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2913446

Product Specific Information

Positive Control: HepG2 cell lysate, Hela cell lysate, mouse brain tissue lysate, mouse cerebellum tissue lysate, A431 cell lysate, rat brain tissue lysate, human placenta tissue, mouse colon tissue, SH-SY5Y, Hela.

Subcellular Location: Nucleus, Cytoplasm.

Target Information

STRAP is a 350 amino acid MAP activator with WD-40 repeats that is localized both in the cytoplasm and the nucleus. It is a ubiquitously expressed protein that acts as an inhibitor of TGF-beta signaling and an important regulator of cell proliferation. Stable expression of STRAP results in activation of the mitogen-activated protein kinase/extracellular signal-regulated kinase pathway and in down-regulation of the cyclin-dependent kinase inhibitor p21 (Cip1), which results in retinoblastoma protein hyperphosphorylation. Upregulation of STRAP might play a role in tumor development as an early event for colorectal cancers. It is the first component of the U snRNP assembly machinery that associates with SMN complex in a compartment-specific way and plays a crucial role in the intracellular distribution of SMN1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.