Thermo Fisher Scientific ZAP70 Monoclonal Antibody (ZAP70, 2035)
인간 ZAP70 단백질에 특이적인 마우스 단클론 항체로, IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 사용 가능. 단백질 A/G로 정제된 액상 형태이며, 무보존제 PBS 버퍼에 저장. T세포 및 NK세포 신호전달 연구에 적합.
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Thermo Fisher Scientific ZAP70 Monoclonal Antibody (ZAP70, 2035)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ZAP70, 2035 |
| Immunogen | Recombinant fragment of human ZAP70 protein (around aa 247–382) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Antibody is tested in direct ELISA (use at 2–4 µg/mL for coating).
- For immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase and a member of the Syk family. It is expressed exclusively in T-cells and natural killer cells and is essential for T-cell receptor activation. Upon TCR engagement, ZAP70 is phosphorylated on tyrosines 292, 315, and 319. Phosphorylation of tyrosines 315 (Vav binding site) and 319 (Lck binding site) enhances ZAP70-mediated lymphocyte signaling, while phosphorylation at tyrosine 292 attenuates activation.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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