Thermo Fisher Scientific Kir7.1 (extracellular) Polyclonal Antibody
Kir7.1(KCNJ13) 단백질의 세포외 영역을 인식하는 Rabbit Polyclonal 항체로, WB, IHC(P), Flow Cytometry 등에 사용 가능. Antigen affinity chromatography로 정제되었으며, 동결건조 형태로 제공. Human, Mouse, Rat 반응성.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Flow Cytometry (Flow) | 1:20 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | (C)EMNGDLEIDHDVPPE, corresponding to amino acid residues 80–94 of rat KCNJ13, Extracellular loop |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.8 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitution: 25 µL, 50 µL, or 0.2 mL double distilled water (DDW), depending on sample size.
- Ships as lyophilized powder at room temperature.
- Upon arrival, store at -20°C.
- Reconstituted solution can be stored at 4°C for up to 1 week.
- For long-term storage, aliquot and store at -20°C.
- Avoid multiple freeze-thaw cycles.
- Centrifuge all antibody preparations before use (10,000 × g, 5 min).
Target Information
Inward rectifier potassium channels preferentially allow potassium influx rather than efflux. Their voltage dependence is modulated by extracellular potassium concentration; as external potassium increases, the voltage range for channel opening shifts positively. Inward rectification is mainly due to blockage of outward current by internal magnesium.
KCNJ13 exhibits low single-channel conductance, low sensitivity to external barium and cesium, and its rectification properties are independent of internal magnesium.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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