Thermo Fisher Scientific DYKDDDDK Tag Monoclonal Antibody (FG4R)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 91 publications 91 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:200-1:500

View 21 publications 21 publications

Flow Cytometry (Flow)

-

View 2 publications 2 publications

ELISA (ELISA)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

3 µg

View 25 publications 25 publications

ChIP assay (ChIP)

-

View 1 publication 1 publication

Peptide Array (Array)

-

View 1 publication 1 publication

Gel Shift (GS)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

FG4R

Immunogen

Synthetic peptide (DYKDDDDK) coupled to KLH.

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• DyLight 488
• DyLight 550
• DyLight 650
• DyLight 680
• DyLight 800 4X PEG
• HRP
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1957945

Product Specific Information

This antibody detects Flag tag from various samples.

similar epitope as the FLAG tag from Sigma

MA1-91878 has been successfully used in immunoprecipitation, Western blot and immunofluorescence applications.

The MA1-91878 immunogen is synthetic peptide (DYKDDDDK) coupled to KLH.

MA1-91878 may be stored at 4ºC short term. Add 0.05% sodium azide if desired. For long term storage, store at -20ºC, avoiding freeze/thaw cycles.

Target Information

DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.