Thermo Fisher Scientific Tag Sequencing Barcode Set 25-48

Thermo Fisher Scientific Tag Sequencing Barcode Set 25-48

The Tag Sequencing Barcode Set 25-48 provides a set of 24 unique barcode adapters specifically designed for optimal performance in Oncomine™ cfDNA assays.
These barcode adapters are compatible with the Ion S5™, Ion PGM™, and Ion Proton™ sequencing systems.
When used with kits utilizing tag sequencing technology, this barcode set enables pooling of up to 24 amplicon libraries for multiplexed next-generation sequencing (NGS) analysis, reducing sequencing cost per sample.

Key Product Features

• Enables multiplexing of numerous amplicon library samples on a single sequencing chip using robust molecular barcodes
• Sequence-optimized and flow-optimized to ensure equal representation of all barcodes in a pool
• Compatible with library construction kits utilizing tag sequencing technology

Multiplexing with Barcode Adapters

Multiplexing with barcoded libraries allows more cost-effective sequencing runs by enabling up to 24 samples per run.
(Or more, if combined with Tag Sequencing Barcode Set 1–24, Cat. No. A31830.)
Each barcode is individually tested to minimize representation bias and ensure consistent performance.

Sequence and Flow Optimization

The barcode adapters contain sequences optimized for equal barcode representation in pooled samples.
They require fewer flows to interrogate, resulting in more economical multiplexed sequencing runs and improved efficiency.

Specifications