Thermo Fisher Scientific T7 Tag Polyclonal Antibody
Rabbit polyclonal antibody recognizing T7 tag sequence (MASMTGGQQMG). Suitable for WB, ICC/IF, ELISA, and IP applications. High specificity and affinity purified. Provided in liquid form, 1 mg/mL concentration, stored at 4°C.
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Thermo Fisher Scientific T7 Tag Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 – 1:30,000 |
| Immunocytochemistry (ICC/IF) | 1:100 – 1:400 |
| ELISA | 1:1,000 – 1:30,000 |
| Immunoprecipitation (IP) | 1 – 4 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | T7 (MASMTGGQQMG) conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Rabbits were immunized with T7 (MASMTGGQQMG) conjugated to KLH. The antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Target Information
The T7 Tag is an 11 amino acid peptide derived from the bacteriophage T7 major capsid protein (gene 10).
T7 cleavage site: MASMTGGQQMG.
Epitope tags provide a method to localize gene products in various cell types, study protein topology and complexes, identify associated proteins, and characterize newly identified or poorly immunogenic proteins when specific antibodies are not available.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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