Thermo Fisher Scientific T7 Tag Polyclonal Antibody, HRP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000 - 1:30,000

-

Immunocytochemistry (ICC/IF)

1:200 - 1:500

-

ELISA (ELISA)

1:10,000 - 1:100,000

-

Product Specifications

Species Reactivity

Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Conjugate

HRP HRP HRP

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 6.8 to 7.4, with 0.2% BSA

Contains

0.05% Pro-Clean 400

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

Rabbits were immunized with T7 (MASMTGGQQMG) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.

Target Information

The T7 Tag is an 11 amino acid peptide derived from the bacteriophage T7 major capsid protein, gene 10. T7 cleavage site: MASMTGGQQMG. Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.