Thermo Fisher Scientific Nicotinic Acetylcholine Receptor beta 4 (CHRNB4) (extracellular) Polyclonal Antibody
Rabbit polyclonal antibody targeting extracellular domain of Nicotinic Acetylcholine Receptor beta 4 (CHRNB4). Validated for WB, IHC(F), ICC/IF. High specificity, antigen affinity-purified, lyophilized form for stable storage. Suitable for human, mouse...
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CYEGVNILRIPAKR, corresponding to amino acid residues 95–108 of rat nAChR beta 4 (Extracellular, N-terminus) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.8 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Reconstitution: Add 50 µL or 0.2 mL double distilled water (DDW), depending on sample size.
The antibody ships as a lyophilized powder at room temperature. Upon arrival, store at -20°C.
Reconstituted solution can be stored at 4°C for up to 1 week. For longer storage, aliquot and keep at -20°C.
Avoid multiple freeze/thaw cycles. Centrifuge antibody preparations before use (10,000 × g, 5 min).
Target Information
After binding acetylcholine, the nicotinic acetylcholine receptor (AChR) undergoes a conformational change that affects all subunits and opens an ion-conducting channel across the plasma membrane.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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