Thermo Fisher Scientific NPHP1 Polyclonal Antibody
Human 및 Mouse 시료에 반응하는 Rabbit Polyclonal 항체로, NPHP1 단백질 검출에 사용됩니다. Western blot 및 Immunocytochemistry에 적합하며, 1 mg/mL 농도의 액상 형태입니다. Affinity chromatography로 정제되었으며, -20°C에서 보관합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000–1:3,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100–1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human NPHP1 (Accession O15259), corresponding to amino acid residues C454–P504 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2853288 |
Product Specific Information
Antibody detects endogenous levels of total NPHP1.
Target Information
Juvenile nephronophthisis type 1 is caused by mutations of NPHP1, the gene encoding for nephrocystin. Nephrocystin interacts with p130Cas (BCAR1), proline-rich tyrosine kinase-2 (PTK2B or Pyk2), and tensin in embryonic kidney and testis, indicating that these proteins participate in a common signaling pathway.
Nephrocystin and p130Cas interact in mammalian cells and both proteins prominently localize at or near sites of cell-cell contact in polarized Madin-Darby canine kidney epithelial cells.
Expression of nephrocystin results in phosphorylation of Pyk2 on Tyrosine 402 as well as activation of downstream mitogen-activated protein kinases, such as ERK1 and ERK2.
Nephrocystin contains a src-homology 3 (SH3) domain, which is highly conserved throughout evolution.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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