Thermo Fisher Scientific Phospho-AMPK alpha-1,2 (Thr183, Thr172) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

-

Immunocytochemistry (ICC/IF)

1:250

View 2 publications 2 publications

Flow Cytometry (Flow)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human AMPK alpha 1 that contains threonine 183 and AMPK alpha 2 that contains threonine 172. The sequence is conserved in human, mouse and rat. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:AMPK alpha-1,2,uniProtId:Q13131-1,ncbiNodeId:9606,antigenRange:183,antigenLength:559,antigenImageFileName:44-1150G_AMPK_alpha-1,2_Q13131-1_Rabbit.svg,antigenImageFileNamePDP:44-1150G_AMPK_alpha-1,2_Q13131-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:AMPK alpha-1,2,uniProtId:P54646-1,ncbiNodeId:9606,antigenRange:172,antigenLength:552,antigenImageFileName:44-1150G_AMPK_alpha-1,2_P54646-1_Rabbit.svg,antigenImageFileNamePDP:44-1150G_AMPK_alpha-1,2_P54646-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco`s PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533585

Target Information

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.