Thermo Fisher Scientific Phospho-AMPK alpha-1,2 (Thr183, Thr172) Polyclonal Antibody
Phospho-AMPK alpha-1,2 (Thr183, Thr172) 폴리클로날 항체로 인간 및 마우스 시료에 반응. Western blot, IHC, ICC/IF 등 다양한 응용 가능. 항원 친화 크로마토그래피로 정제된 액상 형태로 -20°C 보관. 세포 에너지 대사 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 5 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10–1:50 | - |
| Immunocytochemistry (ICC/IF) | 1:250 | View 2 publications |
| Flow Cytometry (Flow) | - | View 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Chemically synthesized phosphopeptide derived from human AMPK alpha 1 (Thr183) and alpha 2 (Thr172). Sequence conserved in human, mouse, and rat. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Dulbecco’s PBS (pH 7.3) with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533585 |
Target Information
The protein encoded by this gene is a catalytic subunit of AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit and non-catalytic beta and gamma subunits. It acts as a key energy-sensing enzyme that monitors cellular energy status. Upon metabolic stress, AMPK is activated and phosphorylates acetyl-CoA carboxylase (ACC) and HMG-CoA reductase (HMGCR), enzymes regulating fatty acid and cholesterol biosynthesis. Mouse studies indicate that this subunit may control insulin sensitivity and maintain myocardial energy homeostasis during ischemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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