Thermo Fisher Scientific SIRP alpha (SIRPA) Monoclonal Antibody (OTI1G4)

Applications

Tested Application

• Western Blot (WB): 1:1,000 dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

SIRP alpha (CD172a, signal-regulatory protein alpha) is a receptor-type transmembrane glycoprotein expressed on myeloid cells such as granulocytes, dendritic cells, macrophages, mast cells, and hematopoietic stem cells.
It acts as a substrate for multiple activated tyrosine kinases (e.g., EGFR, PDGFR, src, insulin receptor) and negatively regulates receptor tyrosine kinase-mediated signaling.

Binding of SIRP alpha to CD47 leads to phosphorylation of ITIM motifs and recruitment of tyrosine phosphatases SHP-1 and SHP-2, influencing immune cell activation and inhibition of phagocytosis.
SIRP alpha plays roles in cellular responses to growth factors, insulin, oncogenes, and adhesion, and is implicated in cancer cell immune evasion through CD47-mediated signaling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.