Thermo Fisher Scientific ENO1 Monoclonal Antibody (KT161)

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:500

Product Specifications

Product Specific Information

This product was used in an Indirect ELISA.

Target Information

Enolase (2-phosphogly-cerate hydrolyase or phosphopyruvate hydrates) is a glycolytic enzyme that catalyzes the dehydration and conversion of 2-phosphoglycerate to phosphoenolpyruvate. It comprises three distinct subunits: alpha, beta, and gamma. Non-neuronal Enolase (ENO1) is an isoform composed of two alpha subunits.
The ENO1 gene also encodes a shorter monomeric structural lens protein, tau-crystallin. ENO1 is a multifunctional enzyme involved in glycolysis, growth control, hypoxia tolerance, and allergic responses. It may also act in the fibrinolytic system by serving as a receptor and activator of plasminogen on cell surfaces such as leukocytes and neurons, and it stimulates immunoglobulin production.

ENO1 is used as a diagnostic marker for various tumors and, in its alpha/gamma heterodimeric form, as a marker for hypoxic brain injury after cardiac arrest. It is also a marker for endometriosis. Antibodies against alpha-enolase are found in sera from patients with cancer-associated retinopathy syndrome (CAR), a progressive blinding disease linked to systemic tumor growth, primarily small-cell carcinoma of the lung and other malignancies.
ENO1 is identified as an autoantigen in Hashimoto encephalopathy (HE), a rare autoimmune disease associated with Hashimoto thyroiditis (HT), which can lead to hypothyroidism due to destructive processes overcoming thyroid replacement capacity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.