Thermo Fisher Scientific SNAP25 Monoclonal Antibody (OTI4G5), TrueMAB
SNAP25 단백질을 인식하는 Mouse Monoclonal Antibody로, WB, IHC, ICC/IF, Flow Cytometry에 적합. 인간 유래 단백질을 면역원으로 제작되었으며, 고순도 Affinity chromatography 정제. Lyophilized 형태로 안정적 보관 가능.
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Thermo Fisher Scientific SNAP25 Monoclonal Antibody (OTI4G5), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4G5 |
| Immunogen | Full length human recombinant protein of human SNAP25 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve an antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
SNAP25 is a t-SNARE protein involved in the molecular regulation of neurotransmitter release.
It plays a role in synaptic function and associates with proteins involved in vesicle docking and membrane fusion.
Additionally, SNAP25 regulates plasma membrane recycling through interaction with CENPF.
Mutations in the SNAP25 gene can result in myasthenic syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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