Thermo Fisher Scientific SNAP25 Monoclonal Antibody (OTI 1E6), TrueMAB
SNAP25 단백질을 인식하는 Mouse monoclonal antibody (Clone OTI 1E6). Western blot, IHC, ICC, Flow cytometry에 적합. Lyophilized 형태로 제공되며, PBS buffer에 8% trehalose 포함. 신경전달 조절 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI 1E6 |
| Immunogen | Full length human recombinant protein of human SNAP25 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, it is recommended to perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
SNAP25 is a t-SNARE protein involved in the molecular regulation of neurotransmitter release. It plays a role in the synaptic function of specific neuronal systems and associates with proteins involved in vesicle docking and membrane fusion. Additionally, SNAP25 regulates plasma membrane recycling through its interaction with CENPF. Mutations in the SNAP25 gene may result in myasthenic syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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