Thermo Fisher Scientific Ki-67 Polyclonal Antibody
Ki-67 단백질을 인식하는 Rabbit Polyclonal Antibody로, 세포 증식 마커 검출에 사용. Western Blot 및 IHC(Paraffin) 적용 가능. 인간 및 랫트 반응성. 항원 친화 크로마토그래피로 정제된 액상 형태, 4°C 단기 보관 및 -20°C 장기 보관 권장.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from human Ki-67 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Breast carcinomas
- Cellular location: Nuclear
Target Information
Ki-67 is a nuclear protein expressed during various stages of the cell cycle, particularly during late G1, S, G2, and M phases. It contains a forkhead associated (FHA) domain that mediates association with euchromatin at the perichromosomal layer, centromeric heterochromatin, and the nucleolus.
Ki-67 exhibits cell cycle-dependent localization: perinucleolar expression at G1, nuclear matrix expression at G2, and chromosomal association during M phase.
Because Ki-67 is absent in G0 cells and increases steadily from G1 through mitosis, it serves as a reliable proliferation marker. Ki-67 antibodies are used to determine the growth fraction in neoplasms, with prognostic value comparable to thymidine-labelling index.
Low Ki-67 index correlates strongly with histologically low-grade tumors. Ki-67 is also routinely employed as a neuronal marker of cell cycling and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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